Core Facilities & Other Resources
General Molecular Biology and Biochemistry
Sorvall T6000 centrifuge and Sorvall RCT-B refrigerated centrifuge, pH meters, electronic balances, fluorometers, autoclave, Bio-Rad suspension array reader, Apollo HP01 and HP10 Mini Oven for Hybridization. Precision Molecular Biology Water Baths, Stuart Scientific Roller Mixer SRT1, SM510 sterilizer, water-bath shakers, fraction collector, speed vacuum, scintillation counter, Fisher Vortex Genie 2.
Separate tissue culture areas, tissue culture hoods and incubators, laminar flow cabinet. New copper lined HERA cell 240i CO2 Incubator (Thermo Scientific).
Plasma and Cell Processing / Storage
Liquid nitrogen tanks, centrifuges, micro-fuges, Beckman Ultracentrifuge, -200 and -800 freezers, large capacity refrigerators, cold room.
RNA and Protein Detection / Quantitation
Bio-Rad SDS-PAGE and Western Blot apparatus, Bio-Rad 2D PAGE system; Roche Real-Time PCR Lightcycler 480, Eppendorf Mastercycler Gradient, high voltage power supplies, gel boxes for DNA/RNA and protein gels, gel dryers, 96-well plate reader for ELISA and protein quantification, Bio-Rad BioplexTM bead array reader, Beckman Coulter DU800 Spectrophotometer, Shimadzu UV-1601PC UV/Vis spectrophotometer, Epoch microplate UV-Vis spectrophotometer to be used for RNA, DNA, protein quantification and for immunoassays, Virsonic Cell Disrupter sonicator, Bio-Rad suspension array reader, and PhosphorImager.
Microscopy / Imaging
Nikon A1 confocal microscope. Nikon inverted microscopes (2) with film, digital and video cameras, Nikon upright microscope with fluorescent and brightfield sources, a transmission electron microscope, darkbox with camera, Kodak Image Station 2000R photography system, darkroom and photographic equipment, four fluorescent micropscopes (one inverted) with digital camera systems, Zeiss electron microscope.
For monocyte identification and Nile Red studies there are 3 flow cytometers: a BD FACSCAN and a BD FACSARRAY Bioanaylzer and a new BD Accuri C6 Flow Cytometer.
HPLC system with Coulochem III (EC) Detector, AKTA FPLC.
One Beckman ultracentrifuge, two Sorvall preparative centrifuges, and several table top centrifuges; microcentrifuges, fixed and swinging bucket rotors.
Analytical balances, spectrophotometers, fluorometers, thermocyclers (traditional and qRT-PCR), -80 freezers, refrigerators, high voltage power supplies, digital imaging system, microplate readers, water batch shakers, speed vacuum concentrator, liquid scintillation counters, icemakers, freezers, dark room, warm rooms, cold rooms, photography equipment, autoclave/dish washing facility, bacterial culture incubators, hybridization incubators, microtomes, and spectrophotometers.
Comparative Medicine Division
Facilitates all animal research. The CMD works within the guidelines and regulations of the United States Department of Agriculture (USDA), Office of Laboratory Animal Welfare (NIH-OLAW), Association for Assessment for Accreditation of Laboratory Animal Care (AAALAC), New York State Department of Health (DOH) and Winthrop University Hospital’s internal code of conduct.
The CMD is comprised of two vivariums which encompass a total of 9,300 square feet of space. The new rodent barrier facility consists of 6,200 square feet and is comprised of four animal holding rooms and a quarantine room. Ancillary space within the barrier include a food and bedding room, flex space room, storage room, waste holding room, pass thru room and dirty/clean cage wash rooms. Equipment includes ventilated cage rack systems, animal changing stations for each holding room, two bulk sterilizers, a rack wash and bottle/cage washers, biosafety cabinets in all procedure rooms, rodent anaesthesia machines, soiled bedding dumping station, ice maker, automatic water bottle and bedding fillers. Pre-barrier floor space includes a locker room, shower, two restrooms, a fully equipped break room, technician desk area, manager’s office and additional storage room. The department has capability to accommodate bio-safety level 2 projects. The second vivarium is a 3,100 square conventional facility which conducts resident training programs for the hospital and has full capability of performing surgery on species ranging from rodents to swine. The operating room is fully equipped with ventilated anaesthesia machines, pulse oximeters, IV perfusions pumps, blood gas machine, cautery and laparoscopic instruments. An autoclave is available for sterilizing surgical packs in the event of sterile surgery.
Our facility has been accredited since 1984 by the Association for Assessment and Accreditation of Laboratory Animal Care (AAALAC International). We also receive yearly site visits from the United States Department of Agriculture and New York State Department of Health. The hospital's National Institute of Health's Assurance is updated every four years through a formal review process. Yearly reports are filed with the above regulatory agencies, which include animal usage numbers, changes to Animal Care and Use Program and additions/deletions to the Institutional Official. Our objective is to furnish humane animal care while providing Principle Investigators the tools needed to accomplish their goals. Supportive and technical assistance is available to those who conduct research in the department. The CMD is fully staffed by a manager, four New York State licensed and AALAS certified veterinary technicians and a part-time weekend caretaker. The hospital contracts a board-certified consulting/attending veterinarian who provides routine coverage for animals housed in the facility.